PacBio HiFi whole genome sequencing of Tribolium castaneum GA2: adult... - SRA

SRX16838510: PacBio HiFi whole genome sequencing of Tribolium castaneum GA2: adult male
1 PACBIO_SMRT (Sequel II) run: 644,471 spots, 7G bases, 4.3Gb downloads

Design: High molecular weight (HMW) DNA extraction was performed from the whole body using the Qiagen MagAttract HMW DNA Kit. DNA shearing was performed using the Diagenode Megaruptor 2 with the 20 kb fragment protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTBell Express Template kit 2.0. The library was size-selected with beads to remove library molecules less than 3 kb in length to generate the final libraries for sequencing. Sequencing was performed on a Sequel II System using Binding Kit v2.0, Sequencing kit v2.0, and SMRT Cell 8M. To target HiFi reads, the library was sequenced using a 30 hour movie time on one SMRTcell. Raw subreads were converted to HiFi data by processing with CCS to call a single high quality consensus sequence for each molecule, using a 99.5% consensus accuracy cutoff.

Submitted by: United States Department of Agriculture

Study: Genome sequencing of Tribolium castaneum GA2 (red flour beetle) adult male principal pseudohaplotype

PRJNA865805 • SRP389799 • All experiments • All runs

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Genome sequencing and assembly of Tribolium castaneum GA2 (red flour beetle) was conducted by the USDA-ARS Ag100Pest Initiative. Principal haplotype

Sample: Tribolium castaneum GA2 (red flour beetle)

SAMN30135407 • SRS14443933 • All experiments • All runs

Organism: Tribolium castaneum

Library:

Name: icTriCast1_PacBio_CCS_low

Instrument: Sequel II

Strategy: WGS